Research Assistant - Molecular/Cellular,
Division of Cardiology

Employer or Institution: University of Cincinnati

Department: Medicine

Contact Person: Deeptankar DeMazumder

231 Albert Sabin Way,
CVC 3923, PO Box 670542,
Cincinnati, OH 45267 USA


Date of Availablity: 3/1/2017

Application Deadline: 6/1/2017

Duration (if applicable): 5 years or longer

Salary: Median salary as per Human Resources and commensurate with position and experience

Job Requirements: QUALIFICATIONS

B.A./B.S. or higher degree in the Biological Sciences or related discipline and experience in Molecular Biology and Genetic Engineering are required skills in Molecular Biology (electrophoresis, RT-PCR, nucleic acid purification) are required knowledge of basic chemistry (determination of molarity, protein concentration, dilutions) and ability to use basic lab equipment, including balances and centrifuges, pH meter, spectrophotometers and microscopes experience with aseptic, sterile techniques and cell culture basic computer skills (word processing, graphics, statistical analysis) demonstrated ability to interact with a diverse group of individuals, learn new techniques quickly, good organizational skills prior experience with patch clamp electrophysiology, small laboratory animals (mice, guinea pig), and genetic engineering (e.g., adenoviral transduction) is preferred

Do you have a Bachelor's (or higher) degree in the life or physical sciences? (Yes/No)
How many years of experience do you have conducting scientific experiments in a basic laboratory setting? (None, 0.5-2 years, >2 years)
Do you have experience with performing DNA, RNA and protein assays, including ELISA, western blots, 2-D gel electrophoresis, and mass spectrometric immunoassays? (Yes/No)
Do you have experience with performing live cellular fluorescence imaging? (Yes/No)
Do you have experience with performing patch clamp electrophysiology? (Yes/No)
Do you have experience with performing genetic manipulations, such as adenoviral-mediated transduction or CRISPR/Cas9 technology? (Yes/No)
Do you have experience with performing aseptic/sterile techniques in small animals? (Yes/No)

Job Description: This position is to support basic/translational research project on Autonomic Remodeling and Modulation Therapy in Heart Failure and Arrhythmic Sudden Death.
This position requires (commensurate with the application for post-doc or research assistant position):
in-depth knowledge in low and high throughput in vitro assays (e.g., RT-PCR, ELISA, Western blotting, luminescent and fluorescent based assay development, proteomics), and in vivo genetic manipulations (e.g., adenoviral-mediated shRNA preparation/transduction) cell culture support (maintaining and plating cell cultures), including handling of animal and human specimens and performance of molecular and cellular biology techniques assisting PI and senior investigators with conducting research utilizing genomics, molecular/cellular biology, fluorescent intracellular dye and patch clamp electrophysiology approaches preparing chemical reagents and biological media, and performing techniques without supervision collaborating with others in the PI’s lab to perform experiments and analyze data maintaining experimental data/records and sharing these with the PI in weekly meetings and presenting research results orally and in writing through conference and journal article publications